Publications

2015

  • Ty E. Adams and James A. Huntington, 2015, Structural transitions during prothrombin activation: On the importance of fragment 2. Biochimie, [Epub ahead of print] Abstract

2014

  • Manja A. Behrens, Timothy J. Sendall, Jan S. Pedersen, Morten Kjeldgaard, James A. Huntington and Jan K. Jensen, 2014, The shapes of Z α1-antitrypsin polymers in solution support the C-terminal domain-swap mechanism of polymerization. Biophys. J., 107(8):1905-12. Abstract

  • Inaki de Diego, Florian Veillard, Maryta Sztukowska, Tibisay Guevara, Barbara Potempa, Anja Pomowski, James A. Huntington, Jan Potempa and F. Xavier Gomis-Ruth, 2014, Structure and mechanism of cysteine peptidase Kgp, a major virulence factor of Porphyromonas gingivalis in periodontitis. J. Biol. Chem., 289(46):32291-302. Abstract

  • Anja Pomowski, F. Isik Ustok and James A. Huntington, 2014, Homology model of human prothrombinase based on the crystal structure of Pseutarin C. Biol. Chem., 395(10):1233-41. Abstract

  • Jonathan Langdon, R.J. Luddington, James A. Huntington and Trevor P. Baglin, 2014, A hereditary bleeding disorder resulting from a premature stop codon in thrombomodulin (p.Cys537Stop). Blood, 124(12):1951-6. Abstract

  • James A. Huntington, 2014, Natural Inhibitors of thrombin. Thromb. Haemost., 111(4):583-9. Abstract

  • Bernhard C. Lechtenberg, Stefan M. Freund and James A. Huntington, 2014, GpIbα interacts exclusively with exosite II in thrombin. J. Mol. Biol., 426(4):881-93. Abstract

2013

  • James A. Huntington, 2013, Thrombin inhibition by the serpins. J Thromb Haemost, Suppl 1:254-64. Abstract

  • Bernhard C. Lechtenberg, Thomas A. Murray-Rust, Daniel J.D. Johnson, Ty E. Adams, Sriram Krishnaswamy, Rodney M. Camire and James A. Huntington, 2013, Crystal structure of the prothrombinase complex from the venom of Pseudonaja textilis. Blood, 122(16):2777-83. Abstract

2012

  • James A. Huntington, 2012, Thrombin plasticity. Biochim Biophys Acta, 1824(1):246-52. Abstract

  • Li Wei and James A. Huntington, 2012, Crystal structures of protease nexin-1 in complex with heparin and thrombin suggest a 2-step recognition mechanism. Blood, 120(2):459-67. Abstract

  • I. Martinez-Martinez, Daniel J.D. Johnson, Masayuki Yamasaki, J. Navarro-Fernandez, A. Ordonez, V. Vicente, James A. Huntington and J. Corral, 2012, Type II antithrombin deficiency caused by a large in-frame insertion: structural, functional and pathological relevance. J Thromb Haemost, 10(9):1859-66. Abstract

  • R.C. Duncan, , F. Mohlin, D. Taleski, T.H. Coetzer, James A. Huntington, R.J. Payne, A.M. Blom, R.N. Pike and L.C. Wijeyewickrema, 2012, Identification of a catalytic exosite for complement component C4 on the serine protease domain of C1s. J Immunol, 189(5):2365-73. Abstract

  • Bernhard C. Lechtenberg, Stefan M. Freund and James A. Huntington, 2012, An ensemble view of thrombin allostery. Biol Chem, 393(9):889-98. Abstract

2011

  • James A. Huntington and Masayuki Yamasaki, 2011, Serpin polymerization in vitro. Methods Enzymol, 501:379-420. Abstract

  • James A. Huntington, 2011, Serpin structure, function and dysfunction. J Thromb Haemost, 9 Suppl 1:26-34. Abstract

  • Masayuki Yamasaki, Timothy J. Sendall, Mary C. Pearce, James C. Whisstock and James A. Huntington, 2011, Molecular basis of α1-antitrypsin deficiency revealed by the structure of a domain-swapped trimer. EMBO Rep, 12(10):1011-7. Abstract

2010

  • Daniel J.D. Johnson, Jonathan Langdown and James A. Huntington, 2010, Molecular basis of factor IXa recognition by heparin-activated antithrombin revealed by a 1.7Å structure of the ternary complex. Proc Natl Acad Sci USA, 107(2):645-50. Abstract

  • James A. Huntington and James C. Whisstock, 2010, Molecular contortionism - on the physical limits of serpin 'loop-sheet' polymers. Biol Chem, 391(8):973-82. Abstract

  • G.A. Silverman, J.C. Whisstock, S.P. Bottomley, James A. Huntington, D. Kaiserman, C.J. Luke, S.C. Pak, J.M. Reichhart and P.I. Bird, 2010, Serpins flex their muscle: I. Putting the clamps on proteolysis in diverse biological systems. J Biol Chem, 285(32):24299-305. Abstract

  • James C. Whisstock, G.A. Silverman, P.I. Bird, S.P. Bottomley, D. Kaiserman, C.J. Luke, S.C. Pak, J.M. Reichhart and James A. Huntington, 2010, Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions. J Biol Chem, 285(32):24307-12. Abstract

  • Bernhard C. Lechtenberg, Daniel J.D. Johnson, Stefan M. Freund and James A. Huntington, 2010, NMR resonance assignments of thrombin reveal the conformational and dynamic effects of ligation. Proc Natl Acad Sci USA, 107(32):14087-92. Abstract

  • P. Kamath, James A. Huntington and S. Krishnaswamy, 2010, Ligand binding shuttles thrombin along a continuum of zymogen- and proteinase-like states. J Biol Chem, 285(37):28651-8. Abstract

  • Masayuki Yamasaki, Timothy J. Sendall, L.E. Harris, Giles M. Lewis and James A. Huntington, 2010, Loop-sheet mechanism of serpin polymerization tested by reactive center loop mutations. J Biol Chem, 285(40):30752-8. Abstract

  • Li Wei, Daniel J.D. Johnson, Ty E. Adams, N. Pozzi, V. De Filippis and James A. Huntington, 2010, Thrombin inhibition by serpins disrupts exosite II. J Biol Chem, 285(49):38621-9. Abstract

2009

  • Ty E. Adams, Li Wei and James A. Huntington, 2009, Molecular basis of thrombomodulin activation of slow thrombin. J Thromb Haemost, 7(10):1688-95. Abstract

  • James A. Huntington, 2009, Slow thrombin is zymogen-like, J Thromb Haemost, Suppl 1:159-64. Abstract

  • Jonathan Langdown, Klara J. Belzar, Wendy J. Savory, Trevor P. Baglin and James A. Huntington, 2009, The critical role of hinge region expulsion in the induced-fit heparin binding mechanism of antithrombin. J Mol Biol, 386(5):1278-89. Abstract

  • James A. Huntington and Li Wei, 2009, Structural insights into the multiple functions of protein C inhibitor. Cell Mol Life Sci. 66(1):113-21. Abstract

  • James A. Huntington Masayuki Yamasaki and Timothy J. Sendall, 2009, New insight into serpin polymerization and aggregation. Prion., 3(1):12-4. Abstract

2008

  • Wei Li and James A. Huntington, 2008, The heparin binding site of protein C inhibitor is protease-dependent. J Biol Chem, 283(51):36039-45. Abstract

  • Masayuki Yamasaki, Wei Li, Daniel J.D. Johnson and James A. Huntington, 2008, Crystal structure of a stable dimer reveals the molecular basis of serpin polymerization. Nature. 455(7217):1255-8. Abstract

  • James A. Huntington, 2008, How Na+ activates thrombin- a review of the functional and structural data, Biological Chemistry,389(8):1025-35. Abstract

  • Wei Li, Ty E. Adams, Jyoti Nangalia, Charles T. Esmon and James A. Huntington, 2008, Molecular basis of thrombin recognition by protein C inhibitor revealed by a 1.6Å structure of the heparin-bridged complex, Proc. Natl. Acad. Sci. USA, 105(12):4661-6. Abstract

  • James A. Huntington, 2008, Structural Insights into the Life History of Thrombin, in Recent Advances in Thrombosis and Hemostasis 2008, editors K. Tanaka and E.W. Davie, Springer Japan KK, Tokyo, pp. 80-106. Abstract

2007

  • Petra Baumgärtner, Margarethe Geiger, Susanne Zieseniss, Julia Malleier, James A. Huntington, Karin Hochrainer, Edith Bielek, Mechthild Stoeckelhuber, Kirsten Lauber, Dag Scherfeld, Petra Schwille, Katja Wäldele, Klaus Beyer, and Bernd Engelmann, 2007, Phosphatidylethanolamine coordinates a selective cell entry route for protein C inhibitor, J. Cell Biol., 179(4):793-804. Abstract

  • Jill C. Rau, L.M. Beaulieu, James A. Huntington, Frank C. Church, 2007, Serpins in thrombosis, hemostasis and fibrinolysis. J Thromb Haemost. 2007 Jul;5 Suppl 1:102-15. Abstract

  • Wei Li, Ty E. Adams, Margareta Kjellberg, Johan Stenflo and James A. Huntington, 2007, Structure of native protein C inhibitor provides insight into its multiple functions. J Biol Chem. 2007 May 4;282(18):13759-68. Abstract

2006

  • Daniel J.D. Johnson, Jonathan Langdown,Wei Li, Stephan A. Luis, Trevor P. Baglin and James A. Huntington, 2006, Crystal structure of monomeric native antithrombin reveals a novel reactive center loop conformation, J Biol Chem, 281(46):35478-86. Abstract

  • Jonathan Langdown, Wendy J. Carter, Trevor P. Baglin and James A. Huntington, 2006, Allosteric activation of antithrombin is independent of charge neutralization or reversal in the heparin binding site, FEBS Lett., 580(19):4709-12. Abstract

  • James A. Huntington, 2006, Shape-shifting serpins—advantages of a mobile mechanism, TiBS, 31(8):427-35. Abstract

  • Ty E. Adams and James A. Huntington, 2006, Thrombin-cofactor interactions: structural insights into regulatory mechanisms, Arterioscler Thromb Vasc Biol , 26 (8):1738-1745. Abstract

  • Daniel J.D. Johnson, Wei Li, Ty E. Adams and James A. Huntington, 2006, Antithrombin-S195A factor Xa-heparin structure reveals the allosteric mechanism of antithrombin activation. EMBO J., 25(9):2029-37. Abstract

  • Wei Li, Louise C. Serpell, Wendy J. Carter, David C. Rubinsztein and James A. Huntington, 2006, Expression and characterization of full-length human huntingtin-an elongated heat repeat protein. J Biol Chem., 281(23):15916-22. Abstract

  • David C. Rubinsztein and James A. Huntington, 2006, Paradoxical aggregation versus oligomerisation properties of mutant and wild-type huntingtin fragments, Exp. Neurol., 199(2):243-4. Abstract

2005

  • Daniel J.D. Johnson, Ty E. Adams and James A. Huntington, 2005, Crystal Structure of wild-type human thrombin in the Na+-free state, Biochem J.,  392(Pt 1):21-8. Abstract

  • James A. Huntington, 2005, Slow thrombin in solution, Biochem J., 390(Pt 2):e1-3. Abstract

  • David A. Lane, Helen Philippou and James A. Huntington, 2005, Directing Thrombin, Blood, 106(8):2605-12. Abstract

  • James A. Huntington, 2005, Heparin activation of serpins, in Chemistry and Biology of Heparin and Heparan Sulfate, editors Garg, Linhardt and Hales, publishers Elsevier Ltd, 367-98. Abstract

  • James A. Huntington, 2005, Molecular recognition mechanisms of thrombin, J Thromb Haemost, 3(8):1861-72. Abstract

  • Wendy J. Carter, Evis Cama and James A. Huntington, 2005, Crystal structure of thrombin bound to heparin, J Biol Chem, 280(4):2745-49. Abstract

2004

  • Denis O’Keeffe, Steven T. Olson, Nijole Gasiunas, John Gallagher, Trevor P. Baglin and James A. Huntington, 2004, The heparin binding properties of heparin cofactor II suggest an antithrombin-like activation mechanism, J Biol Chem, 2004, 279(48):50267-73. Abstract

  • Aiwu Zhou, Penelope E. Stein, James A. Huntington, Pasupathy Sivasothy, David A Lomas, Robin W Carrell, 2004, How small peptides block and reverse serpin polymerization. J.Mol.Biol., 17;342(3):931-41. Abstract

  • J. Corral, J. Aznar, R. Gonzalez-Conejero, P. Villa, A. Minano, A. Vaya, R.W. Carrell, J.A. Huntington and V. Vicente, 2004, Homozygous Deficiency of Heparin Cofactor II. Relevance of P17 Glutamate Residue in Serpins, Relationship With Conformational Diseases, and Role in Thrombosis. Circulation, 110(10):1303-7. Abstract

  • J. Langdown, D.J. Johnson, T.P. Baglin and J.A. Huntington, 2004, Allosteric activation of antithrombin critically depends upon hinge region extension. J Biol Chem. 279(45):47288-97. Abstract

  • Wei Li, Daniel J.D. Johnson, Charles T. Esmon and James A. Huntington, 2004, Structure of the antithrombin-thrombin-heparin ternary complex reveals the antithrombotic mechanism of heparin. Nat. Struct. Mol. Biol., 11(9):857-62. Abstract

  • Wendy J. Carter, Timothy Myles, Craig S. Gibbs, Lawrence L. Leung, and James A. Huntington, 2004, Crystal Structure of Anticoagulant Thrombin Variant E217K Provides Insights into Thrombin Allostery. J Biol Chem. Jun 18;279(25):26387-94. Abstract

  • Javier Corral, James A. Huntington, R González-Conejero, Alec Mushunje, M Navarro, P Marco, V Vicente, RW Carrell. 2004, Mutations in the shutter region of antithrombin result in formation of disulfide-linked dimers and severe venous thrombosis. J Thromb Haemost. 2(6):931-9. Abstract

  • Daniel J.D. Johnson and James A. Huntington, 2004, The influence of hinge region residue Glu381 on antithrombin allostery and metastability., J Biol Chem, 279: 4913-4921. Abstract

2003

  • James A. Huntington and Charles T. Esmon, 2003, The molecular basis of thrombin allostery revealed by a 1.8Å structure of the ‘slow’ form, Structure, Apr;11(4):469-79. Abstract

  • Aiwu Zhou, James A. Huntington, Navraj Pannu, Robin W. Carrell and Randy J. Read, 2003 How vitronectin binds PAI-1 to modulate fibrinolysis and cell migration, Nat Struct Biol. Jul;10(7):541-4. Abstract

  • Daniel J.D. Johnson and James A. Huntington, 2003, Structure of antithrombin in a heparin-bound intermediate state, Biochemistry, Jul 29;42(29):8712-9.  Abstract

  • James A. Huntington, 2003, Mechanisms of glycosaminoglycan activation of the serpins in haemostasis, J Thromb Haemost. Jul;1(7):1535-49. Abstract

  • Robin W. Carrell and James A. Huntington, 2003, How serpins change their fold for better and for worse, Biochem Soc Symp, (70):163-78. Abstract

  • James A Huntington and Trevor P. Baglin, 2003, Targeting thrombin- Rational drug design from natural mechanisms, Trends in Pharm. Sciences, 24(11):589-95. Abstract

  • Alec Mushunje, Aiwu Zhou, Robin W. Carrell and James A. Huntington, 2003, Heparin-induced substrate behavior of antithrombin Cambridge II, Blood, 102(12):4028-34. Abstract

  • Aiwu Zhou, Pennelope E. Stein, James A. Huntington, Robin W. Carrell,  2003 Serpin polymerisation is prevented by a hydrogen-bond network which is centred on His 334 and stabilised by glycerol,  J Biol Chem  Apr 25;278(17):15116-22. Abstract

  • James A. Huntington, Margaretta Kjellberg, Johan Stenflo, 2003, Crystal structure of protein C inhibitor provides insights into hormone binding and heparin activation Structure Feb;11(2):205-15. Abstract

2002

  • Alec Mushunje, Aiwu Zhou, James A. Huntington, J. Conard, Robin W. Carrell, 2002 Antithrombin 'DREUX' (Lys114Glu):  A variant with complete loss of heparin affinity, Thrombosis and Haemostasis, 88, p. 436-443. Abstract

  • Trevor P. Baglin, Robin W. Carrell, Frank C. Church, Charles T. Esmon and James A. Huntington, 2002.  Crystal structures of native and thrombin-complexed heparin cofactor II reveal a multistep allosteric mechanism. Proc. Natl. Acad. Sci. USA, 99:17 p. 11079-11084. Abstract

  • Klara J. Belzar, Aiwu Zhou, Robin W. Carrell, Peter G.W. Gettins, James A. Huntington, 2002.  Helix D elongation and allosteric activation of antithrombin. J Biol Chem. 8;277 (10) p. 8551-8. Abstract

2001

  • James A. Huntington and Robin W. Carrell, 2001.  The serpins: nature's molecular mousetraps.  Science Progress, 84 (Pt 2) p.125-36. Abstract

  • Robin W. Carrell, James A. Huntington, Alec Mushunje, Aiwu Zhou, 2001. The conformational basis of thrombosis. Thromb Haemost  86(1) p.14-22. Abstract

  • James A. Huntington and Penelope E. Stein, 2001.  Structure and properties of ovalbumin. J Chromatography B, 756 p.189-198. Abstract

  • Aiwu Zhou, Robin W. Carrell and James A. Huntington, 2001.  The serpin inhibitory mechanism is critically dependent on the length of the reactive center loop. J Biol Chem. 20; 276 (29) p.27541-7. Abstract

2000

  • James A. Huntington, Randy J. Read and Robin W. Carrell, 2000.  Structure of a serpin-protease complex shows inhibition through deformation. Nature Vol. 407, p.923-926. Abstract

  • James A. Huntington, Airlie McCoy , Klara J. Belzar, Xue Y. Pei, Peter G.W. Gettins, Robin W. Carrell, 2000. The conformational activation of antithrombin. A 2.85Å structure of a fluorescein derivative reveals an electrostatic link between the hinge and heparin binding regions. J Biol Chem. 275(20):15377-83. Abstract

  • Klara J. Belzar, Timothy R. Dafforn, Maurice Petitou, Robin W. Carrell, James A. Huntington, 2000 The effect of a reducing-end extension on pentasaccharide binding by antithrombin.  J Biol Chem. 275(12):8733-41. Abstract

1999

  • Aiwu Zhou, James A. Huntington and Robin Carrell, 1999. Formation of the Antithrombin Heterodimer in vivo and the Onset of Thrombosis. Aiwu Zhou, James A. Huntington, and Robin W. Carrell Blood Vol. 94, p.3388-3396. Abstract

  • James A. Huntington, Navraj S. Pannu, Bart Hazes, Randy J. Read, David A. Lomas and Robin W. Carrell, 1999. A 2.6Å Structure of a Serpin Polymer and Implications for Conformational Disease. Journal of Molecular Biology, Vol. 293, No. 3 p.449-455. Abstract

1998

  • Richard Skinner, Wun-Shaing W. Chang, Lei Jin, Xue Pei, James A. Huntington, Jan-Pieter Abrahams, Robin W. Carrell, and David A. Lomas, 1998. Implications for Function and Therapy of a 2.9Å Structure of Binary-Complexed Antithrombin. Journal of Molecular Biology, Vol. 283. p.9-14. Abstract